As announced before by the RoboCup Federation, the qualified teams of the 2020 competitions are automatically qualified for the 2021 competitions. TC members of the MSL are finalizing the qualification ranking due to qualification material updates of some teams, however, we are happy to announce the list of qualified teams for the 2021 MSL virtual competitions (alphabetically sorted). A total number of eleven teams passed pre-qualification. Points and a complete ranking will be published and announced in near future, however, an alphabetically sorted list is as follow:
| Team Name | Affiliation | Country |
| ASML Falcons | ASML | The Netherlands |
| CAMBADA | University of Aveiro | Portugal |
| DAGOZILLA | Bandung Institute of Technology | Indonesia |
| Hibikino-Musashi | Kyushu Institute of Technology | Japan |
| IRIS | Institut Teknologi Sepuluh Nopember | Indonesia |
| MRL | Qazvin Islamic Azad University | Iran |
| Nubot | National University of Defense Technology | China |
| Robot Club Toulon | University of Toulon | France |
| Tech United Eindhoven | Eindhoven University of Technology | The Netherlands |
| VDL Robot Sports | VDL Enabling Technologies Group in Eindhoven | The Netherlands |
| Water | Beijing Information Science and Technology University | China |
Notice: This post will be updated.